pljm1 egfp lentivirus (Addgene inc)
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pljm1 egfp lentivirus
Pljm1 Egfp Lentivirus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pljm1-egfp/pLJM1-EGFP+(Plasmid+%2319319)/pm41922875-242-11-13
Average 96 stars, based on 401 article reviews
Pljm1 Egfp Lentivirus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pljm1-egfp/pLJM1-EGFP+(Plasmid+%2319319)/pm41922875-242-11-13
Average 96 stars, based on 401 article reviews
pljm1 egfp lentivirus - by Bioz Stars,
2026-10
96/100 stars
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Plasmid Preparation:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Transfection:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Incubation:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Gentle:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Binding Assay:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Western Blot:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Mutagenesis:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Negative Control:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Construct:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Selection:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Two Tailed Test:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Infection:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Confocal Microscopy:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Transduction:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Quantitative Proteomics:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Expressing:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Control:Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Article Snippet: To produce Article Title: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity. Article Snippet: NA Plasmid: pLCKO2:Sirpa (See Table S2 for details) This paper N/A Plasmid: pLentI-PuroR This paper N/A Plasmid: pNheI-EGFP-EcoRI-V1 This paper NA Plasmid: pEGFP-Rab21 This plasmid was a gift from Article Title: Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts Article Snippet: Additionally, the two cell lines were seeded in 100-mm tissue culture dishes and transfected in accordance with the manufacturer’s protocol (4 μg of Article Title: Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice Article Snippet: pLJM1-EGFP , Sancak et al. , pLJM1-EGFP; Article Title: Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Article Snippet: Article Title: Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe Article Snippet: In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Constructs and Sequences Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in Article Title: Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis Article Snippet: Murine NCK1 (NCBI clone NM010878.3), murine NCK2 (NCBI clone NM010879.3) and human EIF4B (NCBI clone NM001300821.2) were subcloned into pMSCVpuro (Clontech) with a N-terminal 3xFLAG tag, FLAG-BirA*-pcDNA5-FRT-TO (Thermo Fisher Scientific, Waltham, MA), pcDNA3.1 (Thermo Fisher Scientific) with a N-terminal 3xFLAG tag (Sigma-Aldrich, St. Louis, MO), or in |